Improving efficiency of cleaning catalysis by spatial organization of enzymes

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Mai-Britt Jensen, Louise E. Horsfall, Susan J. Rosser

Glasgow University, United Kingdom

Esterases catalyse a wide variety of reactions of major industrial interest including the preparation of enantiopure fine chemicals and pharmaceuticals, biopolymer generation and biodiesel production [1][2]. They are also important components of washing detergent formulations constituting a major industrial market for enzymes. Therefore esterase parts which can be used in biotechnology and synthetic biology applications are needed. Molecular scaffolds have previously been used to improve the product titer of multienzyme pathways through co-localisation of sequential enzymes with control of enzyme stoichiometry and spatial organization [3]. By arranging enzymes involved in cleaning catalysis in such a manner, we hope to initially increase the overall efficiency of this reaction as well as stabilizing the enzymes. [1] Bornscheuer UT. FEMS Microbiol Rev. 2002. [2] Panda T, Gowrishankar BS. Appl Microbiol Biotechnol. 2005. [3] Lee H, Deloache WC, Dueber, JE. Metab Eng. 2012